2-amino-6-lower alkyl or aralkyl-3 - cyano-4 5 6 7-tetrahydro thieno-(2 3-c)pyridine

ABSTRACT

THIENOPYRIDINE DERIVATIVES OF THE FORMULA   2-NH2,3-X,6-R-4,5,6,7-TETRAHYDROTHIENO(2,3-C)PYRIDINE   WHEREIN R IS H, ACETYL, ALLYL, 2-PROPYNYL, 3-CHLORO-2HYDROXYPROPYL, CARBOXYMETHYL, ALKYL OF 1 TO 8 CARBON ATOMS, ALKOXYCARBONYL WHEREIN THE ALKOXY MOIETY CONTAINS FROM 1 TO 4 CARBON ATOMS, HYDROXYALKYL WHEREIN THE ALKYL MOIETY CONTAINS FROM 1 TO 4 CARBON ATOMS, ALKOXYCARBONYLALKYL WHEREIN THE ALKOXY AND ALKYL MOIETIES EACH INDEPENDENTLY CONTAINS FROM 1 TO 4 CARBON ATOMS, PHENETHYL, BENZYL, MONO-, DI- OR TRI-SUBSTITUTED BENZYL OR BENZOYL IN WHICH THE SUBSTITUENTS ARE CL, METHYL OR METHOXY, AND X IS CYANO, CARBAMOYL OR ALKOXYCARBONYL WHEREIN THE ALKOXY MOIETY CONTAINS FROM 1 TO 4 CARBON ATOMS, ARE ANALGESIC AND ANTIINFLAMMATORY AGENTS.

United States Patent 015cc 9 v=1. '1. .3,655,682 2-AMlN0-6-LOWER ALKYL R ARALKYL- 3 CYANO 4,5,6,7-TETRAHYDRO THIENO- f.

[2,3 -c]PYRIDINE Michio Nakanishi and Tetsuya Tahara, Nakatsu, Oita,

iI-liroshi Irnamura, Ichikawa, Chiba, and Yutaka Marnyama, Tokyo, Japan, assignors to Yoshitomi Pharmaceutical Industries, Ltd., Osaka, Japan No Drawing. Original application Nov. 29, 1968, Ser. No. 780,218, now Patent No. 3,563,997, dated Feb. 16, 1971. Divided and this application Oct. 28, 1970, Ser.

No. 84,877 r a Int. Cl. cm 31/50 Us. 01. 2 0 2943 0 2 Claims l V ABSTRACT OF THE DISCLOSURE A v Thie nopyridine derivatives of the formula CROSS REFERENCE TO RELATED APPLICATIONS This application is a divisional of our copending application, Ser. No. 780,218 filed Nov. 29, 1968, now US. Pat. No. 3,563,997 granted on Feb. 16, 1971.

This invention relates to novel and therapeutically valuable thienopyridine derivatives.

The novel thienopyridine derivatives of the invention are of the formula:

wherein R is H, acetyl, allyl, 2-propynyl, 3-chloro-2- hydroxypropyl, carboxymethyl, alkyl of 1 to 8 carbon atoms such as methyl, ethyl, propyl, isopropyl, butyl,

isobutyl, sec-butyl, t-butyl, pentyl, hexyl, octyl, alkoxycarbonyl (alkoxy being of 1 to 4 carbon atoms) such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, sec-butoxycarbonyl, tbutoxycarbonyl, hydroxyalkyl (alkyl being of 1 to 4 carbon atoms) such as hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl, alkoxycarbonylalkyl (alkoxy and alkyl each being of 1 to 4 carbon atoms) such as methoxycarbonylbutyl, butoxycarbonyhnethyl, ethoxycarbonylpropyl, propoxycarbonylethyl, phenethyl, benzyl or mono-, dior tri-substituted benzyl or benzoyl in which the substituents are each selected from the group consisting of Cl, methyl and methoxy, and X is cyano, carbamoyl or alkoxycarbonyl (alkoxy being of 1 to 4 carbon atoms) such as methoxycarbonyl, ethoxycarbonyl, propoxycar- 3,655,682 Patented Apr. 11, 1972 g 2 bonyl, iso-propoxycarbonyl, butoxycarbonyl, iso-butoxycarbonyl, sec-buto'xycarbonyl, t-butoxycarbonyl.

The compounds of Formula I are produced by reactmg a compound of the formula with a compound of the formula XCH CN n1 and sulfur in the presence of an organic base, optimally a secondary amine.

Suitable secondary amines, are, for example, dimethylaimine, diethylamine, morpholine, pyrrolidine or piperi- The compounds of Formula I are also produced in a modified manner, namely by first reacting a compound of Formula H with a secondary amine and then reacting the resulting enamine of the formula (Y is secondary amine residue) with a compound of Formula III and sulfur, or by first reacting a compound of Formula II with a compound of Formula IE and then reacting the compound (V) thus obtained R-N =C/ \CN with sulfur in the presence of a secondary amine.

Moreover, the compounds of Formula -I when R is other than hydrogen are produced by reacting a compound of the formula S with a compound of the formula R Z (VII) wherein R is equivalent to R excluding hydrogen, and Z is halogen (e.g. Cl, Br or I).

The compound of Formula II may preferably be used in the form of salt such as hydrochloride, especially when R is H.

These reactions are carried out in an inert solvent such as methanol, ethanol, benzene and toluene, at room temperature or at an elevated temperature, optionally in the presence of a dehydrating agent such as p-toluenesulfonic acid, or in the presence of a deacidifying agent such as sodium carbonate, potassium carbonate and pyridine.

Thus-produced thienopyridine derivatives represented by Formula I can form acid addition salts with various inorganic or organic acids such as hydrochloric, hydrobromic, sulfuric, nitric, oxalic, maleic, fumaric, tartaric useful as analgesic and anti-inflammatory agents. For example, compounds of Formula I listed below have the following pharmacological properties.

1) Inhibition of carrageenin edema (Donryu rat, male; to 200 grams) observed according to the method 3 described by Charles A. Winter et al. in Proceedings of the Society for Experimental Biology and Medicine, New York, vol. 111, pp. 544-547 (1962).

TABLEI Dose (mg/kg.) Inhibition ED Compound per os (percent) (mg/kg.)

Phenylbutazone 100 15 380 Aminopyrine 250 49 210 A: 2-amino-6 benzyl-S-methoxyearbonyl-4,5,6,7-tetrahydrothieno(2,3

c)pyridine. I

B: 2-amino-60thoxycarhonyl-3-methoxycarbonyl-4,5,6,7-tetrahydrothieno(2,3-c)pyridine.

C d 2-2min0-6-henzyl-3-ethoxycarbonyl-4,5,6,7-tetrahydrothieno(2,3-c) pyn me.

D: 2-amino-6-benzyl-3-ethoxycarbonyl-t,5,6,7-tetrahydrothieno(2,3-0) pyridine hydrochloride.

d G-aeetyl-Z-amino-Ziethoxycarbonyl-4,5,6,7-tetrahydrothieno(2,3-c)

pyri me.

F: 2-amino-6-benzyl-3-eyano-4,5,6,7-tetrahydro-thieno(2,3e)pyridine.

G: 2-amino-G-butyl-3ethoxyearbonyl-4,5,6,7-tetrahydro-thieno(2,3-0) pyridine hydrochloride.

Acutetoxicity (LD of 2-amino-6-benzyl-3-ethoxycarbonyl-4,5,6,7-tetrahydro-thieno(2,3 c)pyridine hydrochloride is as follows:

The compounds (I) of the invention and pharmaceutically acceptable acid addition salts thereof can be administered safely per se as analgesics and anti-inflammatory agents or in the form of a pharmaceutical composition in admixture with a suitable and conventional carrier or adjuvant, administrable orally or by way of injection, without causing harm to the host.

The pharmaceutical composition can take the form of tablets, granules, powders, capsules etc., for oral administration; or of injectable solution for subcutaneous or intramucular administration; or of cream, ointment, suppository, etc. for topical administration.

Among these preparations, forms of tablets, granules, powders, and capsules are especially preferable.

The choice of carrier is determined by the preferred form of administration, the solubility of. the compounds and standard pharmaceutical practice. The following are examples of formulations when a compound of the present invention is administered for the pharmaceutical pur- 3D poses. (2) Eifect on the reaction-threshold to the carrageenin inflamed rats paw (-Donryu rat, male; to 180 grams) (A) 25 mg. capsule: Mg. minutes after oral administration of the compound Compound I 25 (I) observed according to the method described in Lactose 90 Archives Internationales de Pharmacodynamie et de Starch 34 Therapie vol. 111, 409-419 (1957). Magnesium stearate 1 TABLE 2 Reaction-threshold (mm. Hg)

' Dose Inflarned N on-inflamed ITao Compound (mg/kg.) paw paw (mg/kg.)

100 203.6 210.4 a: a 1 .2 C 100 174.0 165.6 47

250 208.5 222.0 6 78.4 121.2 1 honylbutnzone 88 {E13 E22 2 250 167.6 134.4 0 66.0 141.6 Mefeuamie acid 88 Egg $218 4 Compound designation is tho same as in Table l.

1T; is the dose required to cause increase of the reaction-threshold by 50 111111. Hg.

Water (a sufiicient quantity to make 1 kilogram).

(D) 1% ointment G. Compound I White va seline A--- 890 Solid parafiin 30 Beeswax 20 Cetostearyl alcohol (B.P. 1963, p. 148) 50 (E) 100 mg. suppository:

' Compound I-100 mg.

Witespol -E751.4 g. Witespol H0.5 g.

Product of Chemische Werke Witten, Mixture of the triglycerides'of natural saturated vegetable fatty acids of the chain length of C12-C1B, containing a quantity of partial glycerides, registered trade names.

Usual daily'dose of the Compound (I) or salt thereof, lies in the range of about 25 to about 300 milligrams more practically about 100 to about 200 milligrams per human adult. Thus, in case of capsules each containing 25 milligrams of compound (I) or salt thereof, one to twelve capsules per day are administered.

These compositions are'especially useful for the treatment of pain, redness andswelling due to post-operative pain, unrological disease ordental disease.

In the following illustrative examples, g. stands for gram(s) and ml. for "milliliter(s).

EXAMPLE 1 A solution of 18.9 g. of 1-benzyl-4-piperidone, 10.0 g. of methyl cyanoacetate, 3.4 g. of powdery sulfur and 10.0 g. of morpholine in 60 ml. of methanol is heated moderately under reflux for. about minutes to dissolve the powdery sulfur. The mixture is heated under reflux for one further hour to complete the reaction. On standing at room temperature, the mixture yields a precipitate. The precipitate iscollected by filtration, washed well with methanol, and recrystallized from methanol to give 27.3 g. (91% yield) of 2-amino-6-benzyl-3-methoxycarbonyl- 4,5,6,7-tetrahydro-thieno(2,3-c)-pyridine as almost colorless needles melting at 124 to 126 C.

EXAMPLE 2 A solution of 22.3 g. of 1-p-chlorobenzyl-4-piperidone, 113g. of-ethyl cyanoacetate, 3.3 g. of powdery sulfur and 10 ml. of diethylamine in 70 ml. of ethanol is heated at 40 C. for, 30. minutesandfurther heated under reflux for 30 minutes. On standing at room temperature, the mixture yields a precipitate. The latter is collected by filtration and recrystallized from ethanol 'to give 30.2 g. (87%) of 2 amino 6-p-chlorobenzyl-3-ethoxycarbonyl-4,5,6,7- tetrahydro-thieno(2;3-c)pyridine as pale yellow needles melting at133-to- 134 C.

A solution of 1.0 g. of the product in 2 ml. of'ethanol is added to 20 ml. of a 10% hydrochloric acid solution. The precipitate crystals are collected by filtration and recrystallized from a 70% aqueous ethanol to give 0.95 g. of the hydrochloride as colorless needles melting at 225 .to 228. C. with decomposition.

EXAMPLE 3 ofmalononitrile, 3.4 g. of sulfur and 9.0 g. of morpholine in 50 ml. of ethanol is heated under reflux for 2 hours. After cooling, the precipitated crystals are collected by filtration and recrystallized from ethanol to give 24.0 g. (93%) of 2-amino-6-benzyl-3-cyano-4,5,6,7-tetrahydrothieno(2,3-c)pyridine as pale yellow prismatic crystals melting at 149 to 152 C.

Its yellow prismatic hydrochloride melts at 238 C. to 240 C. with decomposition.

EXAMPLE 4 A mixture of 22.6 g. of 1-methyl-4-piperidone, 11.3 g. of ethyl cyanoacetate, 1.6 g. of ammonium acetate, 2.4 g. of glacial acetic acid and 70 ml. of benzene is heated under reflux for 3 hours in a flask connected with a condenser provided with a water-removing adapter, to remove 3.8 ml. of water. After cooling, the mixture is washed wtih ml. of a cold 10% aqueous potassium carbonate solution, and dried over sodium sulfate. The crude ethyl (1-methyl-4-piperidone)cyanoacetate remaining after the benzene removal in vacuo is dissolved in 100 ml. of ethanol. To the ethanolic solution are added, 6.6 g. of sulfur and 19.0 g. of morpholine and the whole is heated under reflux for 1.5 hours. After the ethanol is distilled ofl in vacuo, 20% aqueous ethanol is added to the residue. The precipitated crystals are collected by filtration and recrystallized from 50% aqueous ethanol to give 40.1 g. (80.2%) of Z-amino-3-ethoxy-carbonyl-6- methyl 4,5,6,7 tetrahydro-thieno(2,3-c)-pyridine containing 16 molecule of water of crystallization as pale yellow prismatic crystals melting at 103 to 105 C.

Its hydrochloride in the form of colorless needles, recrystallized from 80% aqueous ethanol, melts at 239 to 241 C. with decomposition.

EXAMPLE 5 A solution of 9.4 g. of l-benzyl-4-piperidone, 7.0 g. of butyl cyanoacetate, 1.7 g. of sulfur and 4.5 g. of morpholine in 30 ml. of ethanol is heated under reflux for 2 hours. The mixture is allowed to stand overnight in an icebox, whereby crystals precipitate out. They are collected by filtration, washed with cold ethanol, and recrystallized from ethanol to give 15.2 g. (88.5%) of 2- amino-G-benzyl-3-butoxycarbonyl 4,5,6,7 tetrahydrothieno(2,3-c)pyridine as colorless prismatic crystals melting at 60 to 64 C. with decomposition.

Its hydrochloride in the form of pale yellow needles melts at 214 to 216 C.

EXAMPLE 6 A mixture of 14.1 g. of l-acetyl-piperidone, 9.0 g. of morpholine, 0.2 g. of p-toluenesulfonic acid and 50 ml.

of benzene is heated under reflux for 8 hours in a flask connected with a condenser provided with a water-removing adapter, to remove 1.8 ml. of water. After filtering, the benzene is distilled off in vacuo, and the remaining brown viscous crude 1-acetyl-4-morpholino-1,2, 5,6-tetrahydropyridine is dissolved in 50 ml. of ethanol, and 11.0 g. of ethyl cyanoacetate and 3.2 g. of sulfur are added. The whole is stirred at room temperature for 20 minutes and then heated under reflux for 30 minutes to complete the reaction. Cooling to room temperature affords precipitates, which are collected by filtration and recrystallized from ethanol to give 20.2 g. (76%) of 6-acetyl-2-amino-3-ethoxycarbonyl 4,5,6,7- tetrahydro-thieno(2,3-c) pyridine as colorless needles melting at 148 to 151 C.

EXAMPLE 7 A mixture of 8.5 g. of 4-piperidone, 11.3 g. of ethyl oyanoacetate, 3.2 g. of powdery sulfur, 30 ml. of ethanol and 9ml. of morpholine is heated with stirring at 40 C. for 30 minutes and then at 60 C. for 20 minutes, to dissolve the sulfur. After cooling to room temperature, crystals precipitated are collected by filtration and 'recrystallized from ethanol to give 11.6 g. (51.3%) of 2- amino 3 ethoxycarbonyl 4,5,6,7 tetrahydro thieno- (2,3-c)pyridine as colorless needles melting at 119 to 120 C.

Its hydrochloride melts at 249 C. to 251 C. with decomposition.

EXAMPLE 8 A mixture of 13.6 g. of 4-piperidone hydrochloride, 11.3 g. of ethyl cyanoacetate, 3.3 g. of sulfur, 100 ml. of ethanol and 9 ml. of morpholine is heated with stirring at 60 C. for 20 minutes, the sulfur dissolving and needles beginning to precipitate. The mixture is heated for further minutes and then cooled to room temperature. The precipitated crystals are collected by filtration and recrystallized from 70% aqueous ethanol to give 20.2 g. (76.5%) of 2-amino-3-ethoxycarbonyl-4,5,6,7 tetrahydro-thieno(2,3-c)pyridine hydrochloride as colorless needles melting at 249 to 251 C. with decomposition.

EXAMPLE 9 A mixture of 13.6 g. of 4-piperidone hydrochloride, 14.1 g. of butyl cyanoacetate, 3.2 g. of sulfur, 9 ml. of morpholine and 150 ml. of ethanol is heated with stirring at 40 to 50 C. for about 30 minutes, to dissolve the sulfur. On standing at room temperature, crystals precipitate. They are collected by filtration and recrystallized from 90% aqueous ethanol to give 21.0 g. (72%) of 2- amino 3 butoxycarbonyl 4,5,6,7 tetrahydro-thieno- (2,3-c)pyridine hydrochloride as colorless needles melting at 223 to 225 C. with decomposition.

Its free base in the form of colorless needles melts at 90 to 93 C.

EXAMPLE 10 A mixture of 13.6 g. of 4-piperidone hydrochloride, 6.6 g. of malononitrile, 3.2 g. of sulfur, 9 ml. of morpholine and 150 ml. of ethanol is heated with stirring at 50 C. for 30 minutes, the sulfur dissolving and crystals beginning to precipitate. On standing at room temperature, crystals precipitate. They are collected by filtration and recrystallized from 50% aqueous ethanol to give 14.5 g. (63%) of 2-amino-3-cyano 4,5,6,7 tetrahydro-thieno(2,3-c)pyridine hydrochloride as pale yellow prismatic crystals melting at 260 to 263 C. with decomposition.

EXAMPLE 11 A mixture of 22.6 g. of 2-amino-3-ethoxycarbonyl-4,5, 6,7-tetrahydro-thieno(2,3-c)pyridine, 9.5 g. of 3-chloropropanol, 12.0 g. of potassium carbonate and 100 ml. of ethanol is heated under reflux for 24 hours. After cooling, the reaction mixture is filtered to remove insoluble matter, and 20 ml. of 29% ethanolic hydrochloric acid is added to the filtrate. Crystals precipitated are collected by filtration by means of suction, washed with ethanol, and recrystallized from 80% ethanol to give 22.8 g. of 2- amino-3-ethoxycarbonyl-6-(3 hydroxypropyl) 4,5,6,7- tetrahydro-thieno(2,3-c)pyridine hydrochloride as colorless needles melting at 230 to 231 C. with decomposition.

EXAMPLE 12 Monochloroacetic acid (4.8 g.) is added to a solution of 2.0 g. of sodium hydroxide in 100 ml. of 95% ethanol, and the whole is well stirred to form a suspension of sodium monochloroacetate. To the susepnsion are added 10.5 g. of 2-amino-3-ethoxycarbonyl-4,5,6,7-tetrahydrothieno(2,3-c)pyridine and 7 g. of potassium carbonate. The mixture is heated under reflux for 6 hours. After cooling, the reaction mixture is filtered to remove insoluble matter, and the filtrate is adjusted to pH 7 with 200 ml. of 10% hydrochloric acid. The resulting precipitates are collected by filtration by means of suction, and recrystallized from dimethylformamide to give 8.1 g. of Z-amino- 6-carboxymethyl-3-ethoxycarbonyl 4,5,6,7 tetrahydrothieno(2,3-c) pyridine as colorless powder melting at 250 to 254 C. with decomposition.

In the same manner as in the above examples, the 01- 8 lowing thieno(2,3-c) pyridine derivatives are also produced:

13) 2 amino-6-ethoxycarbonyl-3-methoxycarbonyl-4, 5,6,7-tetrahydro-thieno(2,3-c)pyridine as colorless prismatic crystals melting at 147 to C.;

(14) 2-amino-6-cyclohexyl-3-ethoxycarbonyl 4,5,6,7- tetrahydro-thieno(2,3-c)pyryidine as pale yellow needles melting at 87 to 90 C., and its hydrochloride as colorless needles melting at 232 to 235 C. with decomposition;

15 2-amino-3,6-diethoxycarbonyl-4,5,6,7-tetrahydrothieno(2,3-c)pyridine as colorless prismatic crystals melting at 142 to 144 C.;

(16) 2-amino-6-butyl-3-ethoxycarbonyl-4,5,6,7-tetrahydro thieno(2,3 c)pyridine hydrochloride as colorless needles melting at 182 to 186 C. with decomposition;

(17) 2 amino-6-benzyl-3-ethoxycarbonyl-4,5,6,7-tetrahydro-thieno(2,3-c)pyridine as colorless needles melting at 112 to 113 C., and its hydrochloride as colorless needles melting at 234 to 235 C. With decomposition;

18) 2-amino-6-p-chlorobenzoyl-3-ethoxycarbonyl-4,5, 6,7-tetrahydro thieno(2,3-c) pyridine as colorless prismatic crystals melting at 215 to 219 C.;

19) 2-amino-3-ethoxycarbonyl-6-phenethyl-4,5,6,7-tetrahydro-thieno(2,3-c)pyridine hydrochloride as colorless needles melting at 214 to 215 C. with decomposition;

(20) 6 allyl-2-amino-3-ethoxycarbonyl-4,5,6,7-tetrahyhydro-thieno(2,3-c) pyridine melting at 71 to 74 C., and its hydrochloride melting at 234 to 236 C. with decomposition;

(21) 2-amino-3-ethoxycarbonyl-6-(2-propynyl) 4,5,6, 7-tetrahydro-thieno(2,3-c)pyridine melting at 70 to 73 C., and its hydhochloride melting at 237 C. with decomposition;

(22) 2-amino-3-ethoxycarbonyl-6-m-methylbenzyl-4,5, 6,7-tetrahydro-thieno(2,3-c)pyridine hydrochloride as pale yellow needles melting at 228 to 231 C. with decomposition;

(23) 2 amino 3 ethoxycarbonyl-6-(2- methoxy-S- methylbenzyl)-4,5,6,7-tetrahydro thieno(2,3-c) pyridine hydrochloride as colorless needles melting at 218 to 220 C. with decomposition;

(24) 2 amino-3-ethoxycarbonyl-6-(3,4,5 trimethoxybenzyl)4,5,6,7-tetrahydro-thieno(2,3-c)pyridine as colorless needles melting at 141 C., and its hydrochloride as colorless needles melting at 228 to 230 C. with decomposition;

(25) Z-amino-3-ethoxycarbonyl-6-octyl-4,5,6,7-tetrahydro-thieno(2,3-c) pyridine hydrochloride as pale yellow needles melting at to 193 C.

(26) 2-amino-3-carbamoyl-4,5,6,7-tetrahydro-thieno(2, 3-c)pyridine melting at 202 to 203 C. with decomposition;

(27) 2-amino-3-carbamoyl-6-methyl-4,5,6,7-tetrahydrothieno(2,3-c) pyridine melting at 186 to 189 C. with decomposition;

(28) 2-amino-6-benzyl-3-carbamoy1-4,5,6,7-tetrahydrothieno(2,3-c)pyridine hydrochloride containing /2 molecule of water of crystallization melting at 222 to 225 C. with decomposition;

(29) 2-amino-3-carbamoyl-6-ethoxycarbonyl 4,5,6,7-. tetrahydro-thieno(2,3-c)pyridine melting at 179 to 182. C. with decomposition;

(30) 2-amino-3-carbamoyl-G-p-chlorobenzoyl 4,5,6,7- tetrahydro-thieno(2,3-c)pyridine melting at 208 to 211 C. with decomposition;

(31) 2 amino-3-ethoxycarbonyl-'6-(2-hydroxybutyl)- 4,5,6,7-tetrahydro-thieno(2,3-c)pyridine melting at 104 to 106 C.;

(32) 2 amino 6 (3-chloro-2-hydroxypropyl) 3- ethoxycarbonyl 4,5,6,7-tetrahydro-thieno(2,3-c)pyridine.

melting at 119 to 12 C.;

(33) 2 amino 3 ethoxycarbonyl-6-(2-ethoxycarbonylethyl) 4,5,6,7 tetrahydro-thieno(2,3-c)pyridine hydrochloride melting at 176 to 181 C. with decomposition;

(34) 6 acetyl Z-amino-3-carbamoyl-4,5,6,7-tetrahydro-thieno(2,3-c)pyridine melting at 248 to 251 C. with decomposition;

(35) 2 amino 6-butyl-3-carbamoy1-4,5,6,7-tetrahydro-thieno(2,3-c)pyridine hydrochloride containing /3 molecule of water of crystallization melting at 167 to 168 with decomposition;

(36) 2 amino 3-carbamoyl-6-(2-propynyl)-4,5,6,7- tetrahydro-thieno(2,3-c)pyridine hydrochloride melting at 219 to 220 C. with decomposition;

(37) 2 amino 6-a1lyl-3-carbamoy1-4,5,6,7-tetrahydro-thieno(2,3-c)pyridine hydrochloride melting at 216 to 229 C. with decomposition.

What is claimed is:

1. A compound of the formula References Cited FOREIGN PATENTS 2,004,816 8/1970 Germany 260-294.8

ALAN L. ROTMAN, Primary Examiner US. Cl. X.R. 

